游雄; 錢秀萍; 吳麗燕; 周德清
浙江金華出入境檢驗檢疫局; 上海交通大學(xué)藥學(xué)院; 浙江金華出入境檢驗檢疫局; 浙江金華出入境檢驗檢疫局 金華321001; 上海200240; 金華321001; 金華321001

【中文摘要】 對繡球菌301、302進(jìn)行紫外線誘變處理,以生長速度為指標(biāo)進(jìn)行初篩,再通過深層發(fā)酵進(jìn)行復(fù)篩,綜合分析菌絲體的生長量和胞內(nèi)外多糖的產(chǎn)量,選出301-3、302-2為優(yōu)選菌株。研究不同碳、氮源對誘變株301-3菌絲體生長和多糖產(chǎn)量的影響,認(rèn)為淀粉、麥芽糖是較為合適的碳源;玉米粉和麩皮是較為合適的氮源。對碳氮源正交試驗,確定最佳的培養(yǎng)基組成為:淀粉2.0%、麥芽糖0.4%、玉米粉0.6%、麩皮0.6%。

【英文摘要】 Two strains of Sparassis Crsipa,301 and 302 were treated with UV induced irradiation,then the strains were firstly screened by the index of its growth speed.After submerged liquid fermentation,we re-screed 301-3,302-2 as fine strains when analyzing the amount of their mycelia growth and the yield of intracellular and extracellular polysaccharides.Through the study on the effect that the nitrogen and carbon sources had on strain 301-3,we found that amylum and maltose were appropriate carbon sources for strain 301-3,while corn flour and bran were optimum nitrogen sources.By orthogonal test on the carbon sources and nitrogen sources,we got the suitable ingredients of culture medium for strain 301-3 as follows: amylum2%,maltoes0.4%,corn flour0.6% and bran0.6%.

【中文關(guān)鍵詞】 繡球菌; 紫外誘變; 碳氮源
【英文關(guān)鍵詞】 Sparassis Crispa; UV induced mutagenesis; Nitrogen and carbon source

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2006年03期 【DOI】CNKI:SUN:ZSYJ.0.2006-03-014