鄭海松; 陳明杰; 譚琦; 尚曉冬; 潘迎捷
南京農(nóng)業(yè)大學(xué)資環(huán)學(xué)院微生物系; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 南京210095 ; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 上海201106 ; 上

【中文摘要】 對(duì)目前中國(guó) 7個(gè)主要香菇栽培品種 :蘇香、2 6、939、135、9311、申 10和 74 0 2的 2 5srRNA基因中的D1/D2片段進(jìn)行了DNA序列測(cè)定 ,根據(jù)DNA序列測(cè)定結(jié)果對(duì)它們之間的同源性進(jìn)行了分析。結(jié)果表明 ,所測(cè)定的 7個(gè)香菇栽培品種之間的親緣關(guān)系非常近 ,同源性很高。通過(guò)聚類分析 ,根據(jù)香菇之間的親緣關(guān)系 ,可以將這 7個(gè)品種分為 2類 ,即蘇香、939和 9311為一類 ,2 6、申 10、135和 74 0 2為一類。

【英文摘要】 Seven main cultivation strains of Lentinula edodes widely used in China, Shuxiang, 26, 939, 135, 9311, shen10 and 7402, were analyzed in this study. The D1/D2 region of 25s rRNA gene was amplified by PCR and sequenced. Genetic similarities among these strains were analyzed by the results of D1/D2 sequencing. And the results indicated that their genetic relationship was very close. Based on the cluster analysis results of genetic relationship with Clustal method in PCGENE software, they were divided into two groups, one was Shuxiang, 939 and 9311; the other was 26, Shen 10, 135 and 7402. And their phylogenetic tree was established too.

【中文關(guān)鍵詞】 香菇; 遺傳相似性; D1/D2片段
【英文關(guān)鍵詞】 Lentinula edodes; Genetic similarity; D1/D2 region

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2002年01期 【DOI】CNKI:SUN:SYJB.0.2002-01-002