汪虹; 瞿偉菁; 曹群華
上海市農(nóng)業(yè)遺傳育種重點實驗室上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 華東師范大學(xué)生命科學(xué)院; 華東師范大學(xué)生命科學(xué)院 上海201106 ; 上海200062 ; 上海200062

【中文摘要】 研究表明,金耳(Tremellaaurantialba)液體發(fā)酵最適培養(yǎng)基組成為玉米粉2%,蔗糖3%,酵母膏0.1%,KH2PO40.1%,MgSO40.05%;培養(yǎng)基最佳初始pH、250mL三角瓶裝量、接種量、種子菌齡分別是3.5、80mL、10%和120h;用10L發(fā)酵罐發(fā)酵時,最佳培養(yǎng)條件是種子種齡144h、28℃、接種量10%、通風(fēng)量2.5L min。

【英文摘要】 The results of orthogonal test showed that the optimum medium for the mycelial growth of Tremella aurantiabla was composed with 2% corn powder, 3% sucrose, 0.1% yeast extract, 0.1% KH_2PO_4 and 0.05% MgSO_4,and that the optimum initial pH, medium capacity of 250mL Erlenmeyer flask, inoculation rate and spawn age were 3.5, 80mL, 10% and 120h resp.. When the mycelia of Tremella aurantiabla were cultured in 10L fermentor, the optimum spawn age, culture temperature, inoculation rate and ventilation rate for the mycelial growth of Tremella aurantiabla were 144h, 28℃, 10% and 2.5L/min resp..

【中文關(guān)鍵詞】 金耳; 液體發(fā)酵; 正交試驗; 菌絲產(chǎn)量
【英文關(guān)鍵詞】 Trmella aurantialba; Submerged culture; Orthogonal test; Mycelial yield
【基金】上海市高等學(xué)校科研基金資助項目(JG9507)

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2003年04期 【DOI】CNKI:SUN:SYJB.0.2003-04-007